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Product Description
FitAmp Plasma/Serum DNA Isolation Kit DataSheet
The FitAmp™ General Tissue Section DNA Isolation Kit is a complete set of essential components that enables researchers to efficiently isolate DNA in any targeted microscopic tissue area on a slide. It is suitable for isolating tiny amounts of DNA from microdissection samples, fresh tissue sections, formalin-fixed and paraffin-embedded tissues, plasma, serum, body fluids, etc. Depending on sample types, the entire procedure can be completed within 2 hours.
- Fast - Entire procedure can be finished in just 12 mins with consistent isolation conditions.
- Effective -High efficiency of DNA isolation from plasma or serum containing a tiny amount of DNA (as low as 0.1 ng).
- Reliable – Our specially designed F-Spin Columns allow for DNA to be conveniently recovered.
- Safe -Use of non-toxic reagents and no phenol chloroform.
This kit can also be employed as the initial step in DNA methylation quantitation, followed by the use of the MethylFlash Global DNA Methylation 5-mC ELISA Easy Kit, or the MethylFlash Global DNA Methylation 5-hmC ELISA Easy Kit.
Background
Rapid and efficient preparation of DNA is crucial to a successful, DNA-based experiment. DNA samples can be prepared from cells, tissues, and body fluids, and are the most commonly used starting material for the majority of DNA methylation applications and assays. The FitAmp™ Plasma/Serum DNA Isolation Kit ensures ready-to-use DNA no matter what the downstream application is.
Principle & Procedure
This kit simply applies our proprietary DNA isolation buffer to plasma/serum. After treatment with the DNA digestion buffer, the DNA is easily recovered in 8- 20 µl through our specially designed F-Spin Column. DNA is then ready for downstream application.
Starting Materials
Input amount of plasma, serum or body fluids can be as low as 0.1 ng, with the optimal range between 1 ng and 1 µg. This kit can easily yield 1 ng to 4 µg of DNA in spin column format, which can be used for any molecular biology procedures such as PCR, restriction digestion, cloning and sequencing, etc
