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  • pNPP Phosphatase Assay Kit | POPN-01K

    • pNPP Phosphatase Assay Kit | POPN-01K

    NULL506.00

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    SKU:
    65-POPN-01K
    Availability:
    In Stock
    Size:
    1000 Tests
    Storage:
    4 °C
    Ship Temperature:
    Room Temperature
    Shelf Life Mo:
    12 Months
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    Product Description

    pNPP Phosphatase Assay Kit | POPN-01K

     

    Application: For quantitative determination of phosphatase activity.

    Key Features:

    • High sensitivity and wide linear range. The detection limit is generally 3 ng phosphatase or below.
    • Homogeneous and simple procedure. No wash or reagent transfer steps are involved. The assay can be completed within 30 minutes.
    • Robust and amenable to HTS. All reagents are compatible with high-throughput liquid handling instruments.

    Method: OD405nm

    Samples: Protein phosphatases

    Species: All

    Size: 1000 tests

    Detection Limit: 3 ng

    Shelf Life: 12 months

    More Details:

    p-nitrophenyl phosphate (pNPP) is a chromogenic substrate for most phosphatases such as alkaline phosphatases, acid phosphatases, protein tyrosine phosphatases and serine/threonine phosphatases. The reaction yields para-nitrophenol, which becomes an intense yellow soluble product under alkaline conditions and can be conveniently measured at 405 nm on a spectrophotometer. This homogeneous "mix-and-measure" assay involves simply adding a single reagent to the phosphatase and measuring the product formation on an absorbance reader. The assay can be conveniently performed in cuvettes, tubes or multi-well plates at either room temperature or 37°C.

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    Size 1000 Tests
    Storage 4 °C
    Ship Temperature Room Temperature
    Shelf Life Mo 12 Months

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