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Product Description
QuantiChrom™ Glyoxalase I Assay Kit
Application: For quantitative determination of glyoxalase activity. Note: use a UV plate (e.g. 96-well UV plate, cat# P96UV).
Key Features:
- Fast and sensitive. Detection Limit 4 U/L GLO-1 Activity.
- Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Method: OD240nm
Samples: Enzyme preparations or biological samples
Species: All
Size: 100 tests
Detection Limit: 4 U/L
Shelf Life: 12 months
More Details:
GLYOXALASE I (GLO-1), a lactoylglutathione lyase also known as methylglyoxalase, aldoketomutase, ketone-aldehyde mutase, and (R)-S-lactoylglutathione methylglyoxal-lyase, is an enzyme that catalyzes the isomerization of hemithioacetal adducts which are formed in spontaneous reactions between glutathionyl groups and aldehydes. The primary physiological function of glyoxalase I is the detoxification of methylglyoxal, a reactive 2-oxoaldehyde that is cytostatic at low concentrations and cytotoxic at millimolar concentrations.
Glyoxalase I is a target for the development of pharmaceuticals against bacteria, protozoans and human cancer. Simple, direct and automation-ready procedures for measuring GLO-1 activity in biological samples are highly desirable in research and drug discovery.
BioAssay Systems Glyoxalase I assay kit provides a sensitive and convenient method for GLO-1 activity determination. The method involves monitoring the increase in the product of the GLO-1 reaction, S-lactoylglutathione, by measuring the change in absorbance at 240 nm.
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