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Product Description
Biovision | IL-32 (Human) ELISA Kit | E5054-100
Description: Interleukin-32 (IL-32) is a pro-inflammatory cytokine that induces other cytokines involved in inflammation, including tumor necrosis factor (TNF)-α, IL-6 and IL-1β. IL-32 gene consists of eight small exons, and IL-32 mRNA has nine alternative spliced isoforms. It activates typical cytokine signal pathways of NF-kappa-B and p38 MAPK. IL-32 has a crucial role in host defense against pathogens, as well as in the pathogenesis of chronic inflammation and in cancers connected with chronic inflammation. Abnormal IL-32 expression has been linked to several autoimmune diseases, such as rheumatoid arthritis and inflammatory bowel diseases, and in the pathogenesis of type 1 diabetes. BioVision’s IL-32 (Human) ELISA Kit is used for the quantitative detection of IL-32 in serum, plasma, tissue homogenates and other biological fluids. It is based on the principle of sandwich ELISA. The capture antibody is pre-coated on 96-well plates. The stans, test samples and biotin conjugated detection antibody are added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin is added and unbound conjugates are washed away with wash buffer. The HRP enzymatic reaction is detected using TMB-substrate. Finally, an acidic stop solution terminates the enzymatic reaction. The color developed is directly proportional to the amount of IL-32 in the sample.
Alternate Name: IL-32 ELISA Kit; Interleukin 32 ELISA Kit; NK4 ELISA Kit; TAIF ELISA Kit; IL-32 ELISA Kit; IL-32alpha ELISA Kit; IL-32beta ELISA Kit; interleukin-32 ELISA Kit; interleukin-32 theta ELISA Kit; natural killer cell transcript 4 ELISA Kit; Natural killer cells protein 4 ELISA Kit; NK4IL-32delta ELISA Kit; TAIFa ELISA Kit; TAIFb ELISA Kit; TAIFc ELISA Kit; TAIFd ELISA Kit; TAIFIL-32gamma ELISA Kit; Tumor necrosis factor alpha-inducing factor ELISA Kit
Tag Line: A highly sensitive sandwich ELISA kit for the quantitative measurement of IL-32
Summary: N/A
Detection Method: Absorbance is measured at 450 nm
Sample Type: Human Serum, Plasma, Tissue lysates and other biological fluids
Species Reactivity: Human
Applications: The ELISA kit is used for the quantitative detection of IL-32 in serum, plasma, tissue homogenates and other biological fluids
Features & Benefits: Assay Precision; Intra-Assay CV < 8% and Inter-Assay < 10% Recovery Rate: 89-104% for Serum, 85-105% for EDTA plasma and 87-103% for Heparin plasma Highly sensitive and specific Detection range: 15.625-1000 pg/ml Sensitivity: 9.375 pg/ml
