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Product Description
Biovision | IL5RA (Human) ELISA Kit | E5070-100
Description: Interleukin-5 receptor, alpha (IL5RA) also known as CD125 is a heterodimeric cytokine receptor. The IL5RA is a 60 kDa glycosylated protein which binds IL-5, by itself, with low affinity. IL5RA plays a crucial role in the development, maturation, and operation of eosinophils so were the first important therapeutic target of the new drugs. IL5RA is a therapeutic target for hypereosinophilic diseases including allergic inflammations and asthma. IL5RA also plays a key role in eosinophilia maintenance, and relevant therapeutic strategies include the development of antibodies against IL-5 or IL-5α to control eosinophilia. BioVision’s IL5RA (Human) ELISA Kit is used for the quantitative detection of IL5RA in serum, plasma, tissue homogenates and other biological fluids. It is based on the principle of sandwich ELISA. The capture antibody is pre-coated on 96-well plates. The standards, test samples and biotin conjugated detection antibodye added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin is added and unbound conjugates are washed away with wash buffer. The HRP enzymatic reaction is detected using TMB-substrate. Finally, an acidic stop solution terminates the enzymatic reaction. The color developed is directly proportional to the amount of IL5RA in the sample.
Alternate Name: IL-5RA ELISA Kit; CD125 ELISA Kit; IL5R ELISA Kit; CD125 antigen ELISA Kit; CDw125 ELISA Kit; HSIL5R3 ELISA Kit; IL-5 receptor subunit alpha ELISA Kit; IL-5R subunit alpha ELISA Kit; IL-5R-alpha ELISA Kit; interleukin 5 receptor type 3 ELISA Kit; interleukin 5 receptor ELISA Kit; alpha ELISA Kit; interleukin-5 receptor alpha chain ELISA Kit; interleukin-5 receptor alpha subunit ELISA Kit
Tag Line: A highly sensitive sandwich ELISA kit for the quantitative measurement of IL5RA
Summary: N/A
Detection Method: Absorbance is measured at 450 nm
Sample Type: Human Serum, Plasma, Tissue lysates and other biological fluids
Species Reactivity: Human
Applications: The ELISA kit is used for the quantitative detection of IL5RA in serum, plasma, tissue homogenates and other biological fluids
Features & Benefits: Assay Precision; Intra-Assay CV < 8% and Inter-Assay < 10% Sensitivity: 46.875 pg/ml Highly sensitive and specific Detection range: 78.125-5000 pg/ml Recovery Rate: 86-103% for serum, 85-102% for EDTA plasma and 87-105% for heparin plasma
