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Product Description
Biovision | K2007 | Phosphoglycerate Mutase Activity Assay Kit (Colorimetric/Fluorometric) DataSheet
Phosphoglycerate Mutase (PGAM) is an important enzyme in the glycolytic pathway that catalyzes the reversible conversion of 3‐phosphoglycerate to 2‐phosphoglycerate. It plays an important role in regulating glycolysis and anabolic activity to promote cancer cell proliferation. Several studies reported that PGAM is upregulated in many human cancers. Inhibition of this enzyme could lead to tumor cell death thereby making PGAM an attractive therapeutic target. BioVision’s PGAM Activity Assay Kit can be used to detect its activity in biological samples. The kit utilizes the ability of an active PGAM to catalyze the conversion of 3-Phosphoglycerate to 2-Phosphoglycerate, which is subsequently used to generate an intermediate product. The intermediate product then stoichiometrically reacts with an OxiRed™ probe to generate color (OD 570 nm) or fluorescence (Ex/Em = 535/587 nm). This assay kit provides a rapid, simple and sensitive method to detect PGAM activity as low as 0.05 mU in a variety of samples.
Alternate Name : Phosphoglycerate Mutase Activity Assay Kit (Colorimetric/Fluorometric), PGAM assay, PGAM, PGAM1, PGAM Activity Kit, PGAM activity assay, PGAM activity assay kit, PGAM activity kit, Phosphoglycerate Mutase Kit (Colorimetric/Fluorometric), Phosphoglycerate Mutase Activity Assay, Phosphoglycerate Mutase Kit (Colorimetric/Fluorometric), PGAM assay kit, Phosphoglycerate Mutase Activity Assay Kit, Phosphoglycerate Mutase Assay Kit (Colorimetric/Fluorometric), Phosphoglycerate Mutase Assay Kit, Phosphoglycerate Mutase Assay
Tag Line : to detect PGAM activity in biological samples
Summary : Phosphoglycerate Mutase (PGAM) is an important enzyme in the glycolytic pathway that catalyzes the reversible conversion of 3‐phosphoglycerate to 2‐phosphoglycerate. It plays an important role in regulating glycolysis and anabolic activity to promote cancer cell proliferation. Several studies reported that PGAM is upregulated in many human cancers. Inhibition of this enzyme could lead to tumor cell death thereby making PGAM an attractive therapeutic target. BioVision’s PGAM Activity Assay Kit can be used to detect its activity in biological samples. The kit utilizes the ability of an active PGAM to catalyze the conversion of 3-Phosphoglycerate to 2-Phosphoglycerate, which is subsequently used to generate an intermediate product. The intermediate product then stoichiometrically reacts with an OxiRed™ probe to generate color (OD 570 nm) or fluorescence (Ex/Em = 535/587 nm). This assay kit provides a rapid, simple and sensitive method to detect PGAM activity as low as 0.05 mU in a variety of samples.
Detection Method : Absorbance (OD 570 nm) or Fluorescence (Ex/Em = 535/587 nm)
Sample Type : Animal tissues: Liver, muscle, heart etc., Cell culture: adherent or suspension cells
Species Reactivity : All
Applications : Analysis of glycolytic metabolism in different sample types, Mechanistic studies of various cancers, Screening anti-cancer drugs, Measurement of PGAM activity in various tissues/cells
Features and Benefits : Rapid, Sensitive method to detect PGAM activity as low as 0.05 mU in a variety of samples., Simple
