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Product Description
Biovision | E4982 | NRF2 (Human) ELISA Kit DataSheet
Nuclear factor erythroid 2-related factor 2 (NRF2, NFE2L2) is a basic leucine zipper transcription factor that belongs to the family of cap ‘n’ collar transcription factors. It is ubiquitously expressed in all cell types. One of the main functions of NRF2 is to act as a master regulator in cell detoxification and anti-oxidation processes. Under physiological conditions, NRF2 is associated with KEAP1 and localized in the cytoplasm. However, during oxidative stress, NRF2 disassociates from KEAP1 and translocates to the nucleus. Once inside the nucleus, NRF2 binds to the antioxidant responsive elements (ARE) located in the promoter regions of its target genes, inhibits lipid peroxidation and ferroptosis, and thereby promotes cell survival. NRF2 contributes to the pathophysiology of multiple diseases such as cancer, neurodegenerative diseases, Type 1 and 2 diabetes, atherosclerosis, and COVID-19. Therefore, NRF2 could serve as a promising therapeutic target for the treatment of human diseases. BioVision’s NRF2 (Human) ELISA kit quantitatively measures NRF2 in human serum, plasma, and other biological fluids. Test samples, Standards, and Biotinylated Detection antibody are added to the wells pre-coated with capture antibody and then washed with Wash Buffer. HRP-Streptavidin is added to the plate, and post-incubation any unattached conjugates are washed off by Wash Buffer. The HRP enzymatic reaction is detected by the addition of TMB-substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is directly proportional to the amount of NRF2 in the sample or standard.
Alternate Name : NRF2, HEBP1, Nrf-2, IMDDHH, NFE2L2
Tag Line : A Sandwich ELISA kit for quantitative measurement of NRF2 in human serum, plasma, tissue lysates, and other biological fluids
Summary :
Detection Method : Absorbance (450 nm)
Sample Type : Plasmas
Species Reactivity : Human
Applications : Sandwich ELISA kit for quantitative measurement of NRF2 in serum, plasma, cell and tissue lysates, and other biological fluids
Features and Benefits : Assay Precision: Intra-Assay CV and Inter-Assay CV < 10%
