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Product Description
Biovision | Biotin Ligase (BirA), Recombinant | P1674
Description: Biotin-protein ligase (EC 6.3.4.15) activates biotin to form biotinyl 5'-adenylate and transfers the biotin to biotin-accepting proteins. BirA is E coli biotin ligase that biotinylates specifically a lysine side chain within a 15-amino acid acceptor peptide (also known as Avi-tag).
Biomolecule/Target:
Synonyms: BirA, Biotin operon repressor protein; Biotin holoenzyme synthetase; biotin-(acetyl-CoA carboxylase) synthetase
Alternates names:
Taglines: An enzyme that transfers biotin to biotin-accepting proteins
NCBI Gene ID #: 948469
NCBI Gene Symbol: E.Coli
Accession #: P06709
Recombinant: False
Source: E. coli
Purity by SDS-PAGE: ⥠95% by SDS-PAGE
Assay:
Purity:
Assay #2:
Endotoxin Level:
Activity (Specifications/test method): ⥠30 mU/mg
Biological activity:
Results:
Binding Capacity:
Unit Definition: One unit of BirA is defined as the amount of enzyme that catalyzes the biotinylation of 1 µmol of Avi-tagged protein (CD40L-Avi) per minute under the assay conditions, where 50% of CD40L-Avi is biotinylated in 60 min at 30 °C in the reaction buffer containing 5 mM MgCl2, 2 mM ATP, 150 µM biotin and 50 µM substrate in PBS and monitored on an SDSPAGE.
Molecular Weight: 37.5 kDa (with N-terminal His tag)
Concentration:
Appearance: Sterile Filtered colorless solution
Physical form description: Liquid
Reconstitution Instructions:
Amino acid sequence: Amino acids 2 to 321
Handling: Centrifuge the vial prior to opening.
Usage:
