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Product Description
Hepatocyte Growth Factor (HGF) is a multifunctional growth factor which regulates both cell growth and cell motility. It exerts a strong mitogenic effect on hepatocytes and primary epithelial cells. HGF synergizes with Interleukin-3 and GM-CSF to stimulate colony formation of hematopoietic progenitor cells in vitro and may, therefore, also modulate hematopoiesis. HGF Human Recombinant produced in CHO is a heterodimer, non-glycosylated, polypeptide chain consisting an α-chain of 463 amino acids and β-chain of 234 having a total molecular mass of 80.017 kDa. The HGF is purified by proprietary chromatographic techniques.
Biovision | 4510 | HGF, human recombinant DataSheet
Biomolecule/Target :HGF
Synonyms: Scatter Factor (SF), Hepatopoietin (HPTA), HGF, HGFB, F-TCF
Alternates names: Scatter Factor (SF), Hepatopoietin (HPTA), HGF, HGFB, F-TCF
Taglines: A growth factor regulating cell growth, cell motility, and morphogenesis
NCBI Gene ID #: 3082
NCBI Gene Symbol: HGF
Gene Source: Human
Accession #: P14210
Recombinant: Yes
Source: CHO cells
Purity by SDS-PAGE: ≥97%
Assay: SDS-PAGE
Purity: ≥97%
Assay #2: HPLC
Endotoxin Level: N/A
Activity (Specifications/test method): N/A
Biological activity: The EDâ‚…â‚€, calculated by the dose-dependent proliferation of monkey 4MBr-5 indicator cells was found to be 20-40 ng/ml.
Results: 20-40 ng/ml.
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: 80.017 kDa
Concentration: N/A
Appearance: Lyophilized protein
Physical form description: The protein was lyophilized from a concentrated (1mg/ml) solution containing PBS pH 7.4 containing 0.05% Tween 20 and 100 mM L-arginine.
Reconstitution Instructions: It is recommended to reconstitute the lyophilized Hepatocyte Growth Factor (HGF) in sterile 18MΩ-cmâ»Â¹ Hâ‚‚O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
Amino acid sequence: N/A
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans
