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Product Description
Mast/stem cell growth factor receptor Kit (c-Kit), a member of the protein kinase superfamily, Tyr protein kinase family and CSF-1/PDGF receptor subfamily, is also known as piebald trait protein (PBT), p145 c-kit, tyrosine-protein kinase Kit and CD117,which contains five Ig-like C2-type (immunoglobulin-like) domains and one protein kinase domain. CD117 acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, CD117 can activate several signaling pathways. Furthermore, Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B.
Biovision | P1497 | Human CellExp™ CD117 / c-kit, Human Recombinant DataSheet
Biomolecule/Target :
Synonyms: CD117, SCFR, c-Kit, KIT
Alternates names: CD117, SCFR, c-Kit, KIT
Taglines: Tyrosine-protein kinase that acts as cell-surface receptor
NCBI Gene ID #: 3815
NCBI Gene Symbol: KIT
Gene Source: Human
Accession #: P10721
Recombinant: True
Source: HEK 293 cells
Purity by SDS-PAGE: >95%
Assay: SDS-PAGE
Purity:
Assay #2:
Endotoxin Level: Less than 1.0 EU per ug by the LAL method.
Activity (Specifications/test method): Immobilized Human CD117, His Tag at 2 ug/mL (100 uL/well) can bind Biotinylated Human SCF, Avitag,His Tag with a linear range of 0.5-8 ng/mL
Biological activity:
Results:
Binding Capacity:
Unit Definition:
Molecular Weight: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 57.1 kDa. The protein migrates as 66-85 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation
Concentration:
Appearance: Lyophilized protein
Physical form description: Lyophilized
Reconstitution Instructions: Reconstitute in sterile deionized water to the desired protein concentration.
Amino acid sequence: AA Gln 26 - Thr 516
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans
