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Product Description
Derived from proteolytic degradation of complement C5, C5 anaphylatoxin is a mediator of local inflammatory process. C5 precursor is first processed by the removal of 4 basic residues, forming two chains, beta and alpha, linked by a disulfide bond. C5 convertase activates C5 by cleaving the alpha chain, releasing C5a anaphylatoxin and generating C5b (beta chain + alpha' chain). Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled. The C5a anaphylatoxin interacts with C5AR1 and tick complement inhibitor. C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation.
Biovision | P1468 | Human CellExp™ Complement C5, Rat Recombinant DataSheet
Biomolecule/Target :
Synonyms: Complement C5, C5, CPAMD4
Alternates names: Complement C5, C5, CPAMD4
Taglines: Mediator of local inflammatory process
NCBI Gene ID #: 362119
NCBI Gene Symbol: Hmgb1
Gene Source: Rat
Accession #: XP_001079130.2
Recombinant: True
Source: HEK 293 cells
Purity by SDS-PAGE: >95%
Assay: SDS-PAGE
Purity:
Assay #2:
Endotoxin Level: Less than 1.0 EU per ug by the LAL method.
Activity (Specifications/test method):
Biological activity: Immobilized Rat Complement C5 at 2 ug/mL (100 uL/well) can bind Anti-C5a with a linear range of 0.4-12.5 ng/mL
Results:
Binding Capacity:
Unit Definition:
Molecular Weight: This protein carries a polyhistidine tag at the C-terminus. The mature form of Complement C5 is a disulfide-linked heterodimer composed of proteolytically cleaved α and β chain. Each α and β chain has a calculated MW of 76.1 kDa (β chain) and 114.8 kDa (α chain). The protein migrates as 70 kDa (β chain) and 110 kDa (α chain) under reducing (R) condition (SDS-PAGE) due to glycosylation.
Concentration:
Appearance: Lyophilized powder
Physical form description: Lyophilized
Reconstitution Instructions: Reconstitute in sterile deionized water to the desired protein concentration.
Amino acid sequence: Gln 24 - Ala 1688; Predicted N-terminus: Gln 24 (β chain) & Asp 684 (α chain)
Handling: Centrifuge the vial prior to opening.
Usage:
