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Product Description
Glycolate oxidase is a member of the superfamily of the αÂhydroxy acid oxidases (HAO), enzymes that are present in both plants and animals. It catalyzes the FMNÂmediated oxidation of glycolate to glyoxylate and glyoxylate to oxalate with reduction of oxygen to hydrogen peroxide. The coÂfactor, FMN, is tightly bound but not covalently linked to the protein. In humans and other vertebrates, HAOs are found primarily in the peroxisomes of liver, kidney, and pancreas. Three HAOs have been identified in humans. HAOÂ1 is most highly expressed in liver and pancreas and is most active on glycolate, twoÂcarbon substrates. Glycolate oxidase oxidizes glycolic acid to glyoxylate, and can also oxidize glyoxylate into oxalate. These reactions are central to the toxicity of ethylene glycol poisoning. Recently, HAO has been identified as a major contributor to hyperoxaluria, a disorder in which large deposits of calcium oxalate form kidney stones.
Biovision | P1474 | Hydroxyacid Oxidase-1/HAO-1, Human Recombinant DataSheet
Biomolecule/Target :
Synonyms: HAOX1, Glycolate Oxidase, GOX, HAO1, GOX1
Alternates names: HAOX1, Glycolate Oxidase, GOX, HAO1, GOX1
Taglines: Member of the α-Âhydroxy superfamily, oxidizes glycolic acid to glyoxylate.
NCBI Gene ID #: 54363
NCBI Gene Symbol: HAO1
Gene Source: Human
Accession #: Q9UJM8
Recombinant: True
Source: E coli
Purity by SDS-PAGE: >95%
Assay: SDS-PAGE
Purity:
Assay #2:
Endotoxin Level:
Activity (Specifications/test method): >20 mU/mg. Specific activity was measured by its ability to oxidize glyoxylate.
Biological activity:
Results:
Binding Capacity:
Unit Definition: One unit of HAO activity is the amount of enzyme that generates 1.0 umol of H2O2 per minute at pH 7.5 at 25°C.
Molecular Weight: 43 kDa (N-His Tag)
Concentration:
Appearance: Liquid
Physical form description: Liquid
Reconstitution Instructions:
Amino acid sequence: aa 1-370
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans
