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Product Description
LodA is a novel enzyme (EC 1.4.3.20) that catalyzes the chemical reaction:L-lysine + O2 + H2O↔ 6-amino-2-oxohexanoate + NH3 + H2O2. It has antibacterial activity against a wide spectrum of Gram-positive and Gram-negative bacteria including nosocomial isolates of S.aureus and Pseudomonas sp. The antimicrobial activity is due to hydrogen peroxide generated by its lysine oxidase activity. It also has autotoxic activity. Involved in biofilm differentiation; responsible for cell death within microcolonies during biofilm development which is linked to the generation of a phenotypically diverse dispersal population and thus may play a role in colonization. The lysine oxidase is used in the enzymatic assay of L-lysine.
Biovision | P1500 | L-Lysine 6-Oxidase, M. mediterranea DataSheet
Biomolecule/Target :
Synonyms: L-lysine-epsilon-oxidase, LodA, marinocine
Alternates names: L-lysine-epsilon-oxidase, LodA, marinocine
Taglines:
NCBI Gene ID #:
NCBI Gene Symbol: lodA
Gene Source: Marinomonas mediterranea
Accession #: F2JXJ3
Recombinant: False
Source: Marinomonas mediterranea
Purity by SDS-PAGE: >95%
Assay:
Purity:
Assay #2:
Endotoxin Level:
Activity (Specifications/test method): Specific activity is >2 U/mg. The specific activity of Lysine oxidase was measured in the presence of substrate, L-lysine. Rate of H2O2 production was detected by increase in absorbance.
Biological activity:
Results:
Binding Capacity:
Unit Definition: One unit of L-Lysine oxidase is the amount of enzyme that converts L-lysine to 6-amino-2-oxohexanoate and generates 1.0 umol of H2O2 per minute at pH 7.4 at 25 °C.
Molecular Weight: 80.88 kDa
Concentration:
Appearance: Lyophilized powder
Physical form description: Lyophilized
Reconstitution Instructions: Reconstitute in sterile deionized water.
Amino acid sequence:
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans
