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Product Description
SCGF alpha and beta are hematopoietic growth factor that exerts their activity at early stages of hematopoiesis. The SCGFs are non-glycosylated species-specific cytokines that can support growth of primitive hematopoietic cells, and in combination with EPO or GM-CSF, promote proliferation or erthroid or myeloid progenitors, respectively. Recombinant human SCGF-α is a 33.9 kDa polypeptide containing 305 amino acid residues.
Biovision | 7197 | SCGF-α, human recombinant DataSheet
Biomolecule/Target :SCGF-α
Synonyms: Stem Cell Growth Factor-alpha
Alternates names: Stem Cell Growth Factor-alpha
Taglines: Supports early stages of hematopoiesis
NCBI Gene ID #: 4254
NCBI Gene Symbol: SCF
Gene Source: Human
Accession #: P21583
Recombinant: Yes
Source: E. Coli
Purity by SDS-PAGE: ≥98%
Assay: SDS-PAGE
Purity: N/A
Assay #2: HPLC
Endotoxin Level: < 0.1 ng/ug of protein (<1EU/ug).
Activity (Specifications/test method): Determined by its inhibitory effect on the proliferation of TF-1 cells previously grown in media containing GM-CSF. The ED50 for this effect was observed at 2.5 ng/ml.
Biological activity: Determined by its inhibitory effect on the proliferation of TF-1 cells previously grown in media containing GM-CSF. The ED50 for this effect was observed at 2.5 ng/ml.
Results: N/A
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: 33.9 kDa
Concentration: N/A
Appearance: Lyophilized powder
Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized with no additives
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Amino acid sequence: GHGARGAERE WEGGWGGAQE EEREREALML KHLQEALGLP AGRGDENPAG TVEGKEDWEM EEDQGEEEEE EATPTPSSGP SPSPTPEDIV TYILGRLAGL DAGLHQLHVR LHALDTRVVE LTQGLRQLRN AAGDTRDAVQ ALQEAQGRAE REHGRLEGCL KGLRLGHKCF LLSRDFEAQA AAQARCTARG GSLAQPADRQ QMEALTRYLR AALAPYNWPV WLGVHDRRAE GLYLFENGQR VSFFAWHRSP RPELGAQPSA SPHPLSPDQP NGGTLENCVA QASDDGSWWD HDCQRRLYYV CEFPF
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans
