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Product Description
Glucosidase IIβ (K117) polyclonal Antibody | BS2646 | Bioworld
Host: Rabbit
Reactivity: Human,Mouse,Rat
Application: WB IHC IF
Application Range: WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200
Background: Trimming of glucoses from N-linked core glycans on newly synthesized glycoproteins occurs sequentially through the action of Glucosidases I and II in the endoplasmic reticulum (ER) . Glucosidase II is an ER-localized enzyme that contains α and β subunits (Glucosidase IIα and Glucosidase IIβ) . The α and β subunits form a defined heterodimeric complex with a molecular weight about 161 kDa. Glucosidase IIα is the catalyitc core of the enzyme and can function independently of the β subunit. The sequence of Glucosidase IIβ encodes protein rich in glutamic and aspartic acid with a putative ER retention signal (HDEL) at the C terminus. The phosphorylated form of Glucosidase IIβ is localized in the plasma membrane and is highly expressed in FGF stimulated fibroblasts and epidermal carcinoma cells. Glucosidase IIβ was first purified from a human carcinoma cell line as a potential substrate for protein kinase C. Through the HDEL signal at the C-terminus, Glucosidase IIβ retains the complete complex in the ER.
Storage & Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Glucosidase IIβ (K117) polyclonal Antibody detects endogenous levels of Glucosidase IIβ protein.
Molecular Weight: ~ 59, 80 kDa
Note: For research use only, not for use in diagnostic procedure.
Alternative Names: Glucosidase 2 subunit beta; 80K-H protein; Glucosidase II subunit beta; Protein kinase C substrate 60.1 kDa protein heavy chain; PKCSH; PRKCSH; G19P1
Immunogen: Synthetic peptide, corresponding to amino acids 90-130 of Human Glucosidase IIβ.
Conjugate: Unconjugated
Modification: Unmodification
Purification & Purity: The Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Pathway:
