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Product Description
PGR (phospho-S400) polyclonal Antibody | BS64224 | Bioworld
Host: Rabbit
Reactivity: Human,Rat,Mouse
Application: WB
Application Range: WB: 1:500~1:1000
Background: Human progesterone receptor (PR) is expressed as two forms: the full length PR-B and the short form PR-A. PR-A lacks the first 164 amino acid residues of PR-B. Both PR-A and PR-B are ligand activated, but differ in their relative ability to activate target gene transcription. The activity of PR is regulated by phosphorylation; at least seven serine residues are phosphorylated in its amino-terminal domain. Three sites (Ser81, Ser102, and Ser162) are unique to full length PR-B, while other sites (Ser190, Ser294, Ser345, and Ser400) are shared by both isoforms. Phosphorylation of PR-B at Ser190 (equivalent to Ser26 of PR-A) is catalyzed by CDK2. Mutation of Ser190 results in decreased activity of PR, suggesting that the phosphorylation at Ser190 may be critical to its biological function.
Storage & Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: PGR (Phospho-S400) polyclonal Antibody detects endogenous levels of PGR protein only when phosphorylated at Ser400.
Molecular Weight: ~ 98 kDa
Note: For research use only, not for use in diagnostic procedure.
Alternative Names: Progesterone receptor; PR; Nuclear receptor subfamily 3 group C member 3; PGR; NR3C3
Immunogen: Synthetic phosphopeptide derived from human PGR around the phosphorylation site of Serine 400.
Conjugate: Unconjugated
Modification: Phosphorylation
Purification & Purity: The Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Pathway:
