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Product Description
RAD9A monoclonal Antibody | MB0162 | Bioworld
Host: Mouse
Reactivity: Human,Mouse,Rat,Monkey
Application: WB IP
Application Range: WB: 1:500~1000 IP: 1:50~100
Background: Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair.The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex.Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER) .The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3´-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds;endonuclease FEN1 cleavage activity on substrates with double,nick,or gap flaps of distinct sequences and lengths;and DNA ligase I (LIG1) on long-patch base excision repair substrates.The 9-1-1 complex is necessary for the recruitment of C12orf32/RHINO to sites of double-stranded breaks (DSB) occurring during the S phase.RAD9A possesses 3´->5´ double stranded DNA exonuclease activity.Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex.
Storage & Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: This Antibody detects endogenous levels of RAD9A and does not cross-react with related proteins
Molecular Weight: Predicted band size:43KDa Observed band size:55KDa
Note: For research use only, not for use in diagnostic procedure.
Alternative Names: Cell cycle checkpoint control protein; DNA repair exonuclease rad9 homolog A; hRAD 9; hRAD9; Rad 9; RAD 9A; RAD9 (S pombe) homolog; RAD9 homolog A; RAD9 homolog; RAD9A; RAD9
Immunogen: Recombinant full length Human RAD9A.
Conjugate: Unconjugated
Modification: Unmodification
Purification & Purity: The Antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Pathway:
