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Product Description
ZAP70 (4B2) monoclonal Antibody | MB0187 | Bioworld
Host: Mouse
Reactivity: Human
Application: WB IP
Application Range: WB: 1:1000 IP: 1:50~200
Background: The activation of T lymphocytes by antigens is mediated by the T cell receptor (TCR), which is a multisubunit complex assembled from at least six different genes . The TCR subunits include the Ti a and b chains, the CD3 g, d and e chains and a z -containing homodimer or heterodimer . The protein tyrosine kinase ZAP-70 binds to the phosphorylated immunoreceptor tyrosine-base activation motifs (ITAMs) of the TCR z chain through two src-homology (SH2) domains . This binding results in the phosphorylation of ZAP-70 on multiple tyrosine residues, including Tyr292 and Tyr319 . ZAP-70 is autophosphorylated on Tyr292, which is thought to negatively regulate ZAP-70 function in lymphocytes . Alternatively, ZAP-70 is positively regulated by phosphorylation on Tyr319, which mediates the SH2-dependent interaction between Lck and ZAP-70 .
Storage & Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: This Antibody detects endogenous levels of ZAP70 and does not cross-react with related proteins.
Molecular Weight: Predicted band size:70KDa Observed band size:70KDa
Note: For research use only, not for use in diagnostic procedure.
Alternative Names: Tyrosine-protein kinase ZAP-70; 70 kDa zeta-chain associated protein; Syk-related tyrosine kinase; ZAP70; SRK
Immunogen: Purified recombinant human ZAP70 protein fragments expressed in E.coli.
Conjugate: Unconjugated
Modification: Unmodification
Purification & Purity: The Antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Pathway: Hypoxia Signaling,AMPK Signaling Pathway,MTOR signaling,
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