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Product Description
MDM2 (S160) polyclonal Antibody | BS1447 | Bioworld
Host: Rabbit
Reactivity: Human,Mouse,Rat
Application: WB IHC IF
Application Range: WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200
Background: p53 is the most commonly mutated gene in human cancer identified to date. Expression of p53 leads to inhibition of cell growth by preventing progression of cells from G1 to S phase of the cell cycle. Most importantly, p53 functions to cause arrest of cells in the G1 phase of the cell cycle following any exposure of cells to DNA-damaging agents. The MDM2 (murine double minute-2) protein was initially identified as an oncogene in a murine transformation system. MDM2 functions to bind p53 and block p53-mediated transactivation of cotransfected reporter constructs. The MDM2 gene is amplified in a high percentage of human sarcomas that retain wildtype p53 and tumor cells that overexpress MDM2 can tolerate high levels of p53 expression. These findings argue that MDM2 overexpression represents at least one mechanism by which p53 function can be abrogated during tumorigenesis.
Storage & Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: MDM2 (S160) polyclonal Antibody detects endogenous levels of MDM2 protein.
Molecular Weight: ~ 55,90 kDa
Note: For research use only, not for use in diagnostic procedure.
Alternative Names: E3 ubiquitin-protein ligase Mdm2; Double minute 2 protein; Hdm2; Oncoprotein Mdm2; p53-binding protein Mdm2; MDM2
Immunogen: Synthetic peptide, corresponding to amino acids 130-180 of Human MDM2.
Conjugate: Unconjugated
Modification: Unmodification
Purification & Purity: The Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Pathway: Translational Contral elF4 and p70 S6 Kinase,Signaling Pathways Activating p38 MAP KinaseInhibition of Apoptosis,G Protein-coupled Receptors Signaling to MAPK Erk,Cell Cycle G2 M DNA Damage Signaling Pathway,Mitochondrial Control of Apoptosis,MTOR signaling,
