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Product Description
DFFA polyclonal Antibody | BS70023 | Bioworld
Host: Rabbit
Reactivity: Human,Mouse,Rat
Application: WB IF
Application Range: WB 1:500 - 1:2000 IF 1:50 - 1:100
Background: The CED/ICE family of cysteine proteases plays a pivotal role in mediating apoptosis through the proteolysis of specific targets. Among the targets are poly (ADP-ribose) polymerase (PARP), gelsolin, DFF-45/ICAD and the nuclear lamins. PARP is a nuclear protein that is specifically cleaved by CPP32 and Mch2, but not by ICE, into a signature apoptotic fragment. Gelsolin is cleaved by CPP32 to an active form that severs actin filaments in a Ca++-independent manner. In addition to binding actin, gelsolin can form complexes with fibronectin, which may be important for localizing gelsolin to inflammatory sites. DFF-45/ICAD, the subunit of DNA fragmentation factor, is cleaved by CPP32 to generate an active factor that induces DNA fragmentation. The nuclear Lamin A is cleaved by Mch2, but not CPP32. Nuclear Lamin B is fragmented as a consequence of apoptosis by an unidentified member of the ICE family .
Storage & Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: DFFA polyclonal Antibody detects endogenous levels of DFFA protein.
Molecular Weight: ~ 35, 45 kDa
Note: For research use only, not for use in diagnostic procedure.
Alternative Names: DNA fragmentation factor subunit alpha; DNA fragmentation factor 45 kDa subunit; DFF-45; Inhibitor of CAD; ICAD; DFFA; DFF1; DFF45; H13
Immunogen: Recombinant protein of human DFFA.
Conjugate: Unconjugated
Modification: Unmodification
Purification & Purity: The Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Pathway: Immune Cell Signaling,Warburg Effect,Regulation of Microtubule Dynamics,Insulin &Glucose Signaling,G Protein-coupled Receptors Signaling to MAPK Erk,MAPK Erk in Growth and Differentiation Pathway,ErbB HER Signaling,
![[KO Validated] DFFA Polyclonal Antibody](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/500x659/products/295398/455535/sab-signalway-antibody__92073.1641690747__58472.1641691390.gif?c=2 "[KO Validated] DFFA Polyclonal Antibody")
