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Product Description
Calcium assay kit (o-Cresolphtalein-Complexone)
[ Product Description ]
Calcium is the most abundant mineral which accounts for 1.5-2.9% of body weight, and is an important element in the formation of bone and cartilaginous tissues. In the human bodies, 90% of the calcium is present as hydroxyapatite in bone, 1% is in cells, and 0.1% is in the blood. Calcium is known to plays important roles in various physiological functions such as osmotic homeostasis, blood clotting, muscle atrophy, nerve stimulation, and the activation of enzymes. In blood, 45% of calcium is attached to albumin or globulin, 10% is present as phosphate salt, and 45% exist as free ion.
[ Assay principle ]
o-Cresolphthalein Complexone (OCPC) and calcium can form a chelate complex with deep red color. The concentration of calcium can be determined by the absorbance at 570 nm. Although most OCPC-based calcium assay has sigmoid-shaped calibration plot, the calibration plot of this kit is linear and the calcium concentration can be estimated by simple one-point calibration.
[ Performance ]
| 1. Sensitivity: 2. Accuracy: 3. Intra assay variation: 4. Assay range: |
O.D. at 10 mg/dL standard will be 0.5 - 1.0 ±15% of known concentration C.V. is less than 5% 0.2 - 30 mg/dL |
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| [ References: Assay examples ] | ||||||
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