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Product Description
Recombinant Zebrafish VEGF165 Protein, FITC conjugated | VEGF165-1026ZF | Creative BioMart
Bio-activity :
Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is typically 10-30 ng/mL.
Molecular Mass :
The recombinant zebrafish VEGF165 consists of 165 amino acids and has a calculated molecular mass of 19.2 kDa. It migrates as an 22 kDa band in SDS-PAGE under reducing conditions.
Endotoxin :
< 1.0 EU/ μg of the protein as determined by the LAL method.
Purity :
> 90 % as determined by SDS-PAGE
Characteristic :
Disulfide-linked homodimer
Labeled with FITC via amines
Excitation source: 488 nm spectral line, argon-ion laser
Excitation Wavelength: 488 nm
Emission Wavelength: 535 nm
Stability :
Samples are stable for up to 12 months from date of receipt at -70 centigrade.
Storage :
Store it under sterile conditions at -20 to-70 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Storage Buffer :
Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 7.01, 5%-8% trehalose and mannitol.
Reconstitution :
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 μg/μL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.
