Product Description
NPHS1 Polyclonal Antibody | E-AB-12592 | Elabscience
Type: Polyclonal Antibody
Synonyms: CNF, Nephrin, Nephrosis 1 congenital Finnish type, Nephrosis 1, congenital, Finnish type (nephrin), NPHN, NPHN, NPHS 1, Nphs1, Renal glomerulus specific cell adhesion receptor, Renal glomerulus-specific cell adhesion receptor
Application: WB, IHC, ELISA
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Reserch Areas: Cancer, Signal Transduction
Background: This gene encodes a member of the immunoglobulin family of cell adhesion molecules that functions in the glomerular filtration barrier in the kidney. The gene is primarily expressed in renal tissues, and the protein is a type-1 transmembrane protein found at the slit diaphragm of glomerular podocytes. The slit diaphragm is thought to function as an ultrafilter to exclude albumin and other plasma macromolecules in the formation of urine. Mutations in this gene result in Finnish-type congenital nephrosis 1, characterized by severe proteinuria and loss of the slit diaphragm and foot processes.In Western blots, nephrin antibodies generated against the two terminal extracellular Ig domains of recombinant human nephrin recognized a 180-kDa protein in lysates of human glomeruli and a 150-kDa protein in transfected COS-7 cell lysates.
Concentration: 0.5 mg/mL
Storage: Store at -20°C. Avoid freeze / thaw cycles.
Immunogen: Synthetic peptide of human NPHS1
Buffer: PBS with 0.05% sodium azide and 50% glycerol, PH7.4
Purification Method: Affinity purification
Dilution: WB 1:500-1:2000, IHC 1:25-1:100
Clone: N/A
Conjugation: Unconjugated
Molecular Weight(Calculated): 135 kDa
Molecular Weight(Observed): N/A