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![293T [HEK-293T] Cell Line](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/1280x1280/products/904691/1196892/elabscience__13538.1643193164__44410.1643194172__90041.1647951641__83111.1664298182.png?c=2 "293T [HEK-293T] Cell Line")
![293T [HEK-293T] Cell Line](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/100x100/products/904691/1196892/elabscience__13538.1643193164__44410.1643194172__90041.1647951641__83111.1664298182.png?c=2 "293T [HEK-293T] Cell Line")
Product Description
293T [HEK-293T] Cell Line | EP-CL-0005 | Elabscience
Background: This cell line is competent to replicate vectors carrying the SV40 region of replication. It gives high titers when used to produce retroviruses. It has been widely used for retroviral production, gene expression and protein production.
Species: Homo sapiens, Human
Age: Fetus
Tissue: Embryonic kidney
Morphology: Epithelial
Growth Properties: Adherent
Organism: Homo sapiens, Human
Species Cell: Human
application: N/A
Doubling Time: ~24-30 hours
Biosafety: 2
Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Ratio: 1:3-1:6
Renewal: Every 2 to 3 days
![293T [HEK-293T] Cell Line](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/500x659/products/904691/1196892/elabscience__13538.1643193164__44410.1643194172__90041.1647951641__83111.1664298182.png?c=2 "293T [HEK-293T] Cell Line")
![293T [HEK-293T] Cells Complete Medium](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/500x659/products/450494/653576/elabscience__13538.1643193164__44410.1643194172__76355.1648028212.png?c=2 "293T [HEK-293T] Cells Complete Medium")
