Product Description
6T-CEM Cell Line | EP-CL-0008 | Elabscience
Background: This is a 6-thioguanine resistant variant of CCRF-CEM.It is deficient in Hypoxanthine phosphoribosyl transferase(HPRT, HGPRT) and secretes high titers of an immunosuppressive factor.This line may be used as a fusion partner for making human T cell hybridomas.suspensionimmunosuppressive factorCultures can be maintained by addition or replacement of fresh medium. Start cultures at 2×10 exp5 cells/ml and maintain between 1×10 exp5 and 1×10 exp6 cells/ml.Handle as potentially biohazardous material under at least Biosafety Level 2 containment.
Species: Homo sapiens, Human
Age: N/A
Tissue: Peripheral blood
Morphology: Lymphoblast
Growth Properties: Suspension
Organism: Homo sapiens, Human
Species Cell: Human
application: N/A
Doubling Time: N/A
Biosafety: 2
Subculturing: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at the subcultivation ratio of 1:2-1:4.
Ratio: 1:2-1:4
Renewal: 2 to 3 times per week