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Product Description
A-431 Cell Line | EP-CL-0015 | Elabscience
Background: The A-431 cell line was established by D.J. Giard in 1973.
Species: Homo sapiens, Human
Age: 85 years
Tissue: Skin, epidermis
Morphology: Epithelial
Growth Properties: Adherent
Organism: Homo sapiens, Human
Species Cell: Human
application: Transfection host.
Doubling Time: ~80-100 hours
Biosafety: 1
Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Ratio: 1:3-1:6
Renewal: Every 2 to 3 days
