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![A549 [A-549] Cell Line](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/1280x1280/products/904699/1196900/elabscience__13538.1643193164__44410.1643194172__90041.1647951641__15174.1664298184.png?c=2 "A549 [A-549] Cell Line")
![A549 [A-549] Cell Line](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/100x100/products/904699/1196900/elabscience__13538.1643193164__44410.1643194172__90041.1647951641__15174.1664298184.png?c=2 "A549 [A-549] Cell Line")
Product Description
A549 [A-549] Cell Line | EP-CL-0016 | Elabscience
Background: Studies by M. Lieber, et al. revealed that A549 cells could synthesize lecithin with a high percentAge of desaturated fatty acids utilizing the cytidine diphosphocholine pathway.
Species: Homo sapiens, Human
Age: 58 years
Tissue: Lung
Morphology: Epithelial
Growth Properties: Adherent
Organism: Homo sapiens, Human
Species Cell: Human
application: Transfection host.
Doubling Time: ~40 hours
Biosafety: 1
Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Ratio: 1:3-1:6
Renewal: 2 to 3 times per week
![A549 [A-549] Cell Line](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/500x659/products/904699/1196900/elabscience__13538.1643193164__44410.1643194172__90041.1647951641__15174.1664298184.png?c=2 "A549 [A-549] Cell Line")
![A549 [A-549] Cells Complete Medium](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/500x659/products/450504/653586/elabscience__13538.1643193164__44410.1643194172__75804.1648028213.png?c=2 "A549 [A-549] Cells Complete Medium")
