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Product Description
A9 cell line | EP-CL-0018 | Elabscience
Background: This cell line is derived from L929, selected in azauanine. The cell line is sensitive to HAT selection medium, resistant to 8-azaguanine (3 µg/mL) and 6-thioguanine (0.1 mM), and deficient in adenosine phosphoribosyl transferase (APRT) and hypoxanthine phosphoribosyl transferase (HPRT).
Species: Mus musculus, Mouse
Age: male, 100 days old
Tissue: Subcutaneous Connective Tissue; Loose Connective Tissue And Fat ; Normal
Morphology: Fibroblast-Like
Growth Properties: Adherent
Organism: Mus musculus, Mouse
Species Cell: Mouse
application: N/A
Doubling Time: N/A
Biosafety: 1
Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Ratio: 1: 3-1: 4
Renewal: Every 2 to 3 days
