Euro
USD
British Pound
NULL
Product Description
ACHN Cell Line | EP-CL-0021 | Elabscience
Background: Growth is inhibited by human interferon [Hogan, T. F., personal communication].
Species: Homo sapiens, Human
Age: 22 years
Tissue: Kidney, derived from metastatic site: pleural effusion
Morphology: Epithelial
Growth Properties: Adherent
Organism: Homo sapiens, Human
Species Cell: Human
application: ACHN may be of use for antiproliferative studies using human interferons or interferon inducers.
Doubling Time: N/A
Biosafety: 1
Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Ratio: 1:2-1:4
Renewal: 2 to 3 times per week
