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![B-CPAP [BC-PAP; BCPAP] cell line](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/1280x1280/products/904983/1197184/elabscience__13538.1643193164__44410.1643194172__90041.1647951641__76993.1664298249.png?c=2 "B-CPAP [BC-PAP; BCPAP] cell line")
![B-CPAP [BC-PAP; BCPAP] cell line](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/100x100/products/904983/1197184/elabscience__13538.1643193164__44410.1643194172__90041.1647951641__76993.1664298249.png?c=2 "B-CPAP [BC-PAP; BCPAP] cell line")
Product Description
B-CPAP [BC-PAP; BCPAP] cell line | EP-CL-0575 | Elabscience
Background: This cell line was established from the tumor tissue of a 76-year-old woman with metastasizing papillary thyroid carcinoma in 1992.
Species: Homo sapiens, human
Age: Female,76 years
Tissue: Thyroid
Morphology: Epithelial
Growth Properties: Adherent
Organism: Homo sapiens, human
Species Cell: Human
application: N/A
Doubling Time: ~30 hours
Biosafety: 1
Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Ratio: 1:2-1:5
Renewal: Every 2 to 3 days
![B-CPAP [BC-PAP; BCPAP] cell line](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/500x659/products/904983/1197184/elabscience__13538.1643193164__44410.1643194172__90041.1647951641__76993.1664298249.png?c=2 "B-CPAP [BC-PAP; BCPAP] cell line")
![B-CPAP [BC-PAP; BCPAP] Cells Complete Medium](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/500x659/products/450803/653885/elabscience__13538.1643193164__44410.1643194172__00845.1648028247.png?c=2 "B-CPAP [BC-PAP; BCPAP] Cells Complete Medium")
