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Product Description
B16-F1 cell line | EP-CL-0318 | Elabscience
Background: The B16-F1 cell line was derived from B16-F0 cell line of the skin of Mouse melanoma C57BL/6J and can be used for melanin production.
Species: Mus musculus, Mouse
Age: N/A
Tissue: Skin
Morphology: Epithelial
Growth Properties: Adherent
Organism: Mus musculus, Mouse
Species Cell: Mouse
application: N/A
Doubling Time: N/A
Biosafety: 1
Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Ratio: 1: 3-1: 4
Renewal: Every 2 to 3 days
