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Product Description
BV2 Cell Line | EP-CL-0493 | Elabscience
Background: N/A
Species: Mus musculus, Mouse
Age: N/A
Tissue: Brain
Morphology: Morphology microglial
Growth Properties: Mixed, Adherent And Suspension
Organism: Mus musculus, Mouse
Species Cell: Mouse
application: N/A
Doubling Time: N/A
Biosafety: 1
Subculturing: Remove and discard culture medium. These cells grow as a mixture of floating and adherent cells. Sometimes many cells are floating, they can be harvested by centrifugation of medium instead of discarding it. Add 1.0 to 2.0 mL of 0.25% (w/v) Trypsin-0.53mM EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C.
Ratio: 1:2-1:4
Renewal: Every 2 to 3 days
