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Product Description
C2C12 Cell Line | EP-CL-0044 | Elabscience
Background: The C2C12 cell line differentiates rapidly, forming contractile myotubes and producing characteristic muscle proteins.Treatment with bone morphogenic protein 2(BMP-2) cause a shift in the differentiation pathway from myoblastic to osteoblastic.Tested and found negative for ectromelia virus(mousepox).
Species: Mus musculus, Mouse
Age: N/A
Tissue: Muscle
Morphology: Myoblast
Growth Properties: Adherent
Organism: Mus musculus, Mouse
Species Cell: Mouse
application: Transfection host.
Doubling Time: N/A
Biosafety: 1
Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Ratio: 1:2-1:4
Renewal: Every 2 to 3 days
