Euro
USD
British Pound
NULL
Product Description
Calu-1 cell line | EP-CL-0053 | Elabscience
Background: This cell line is derived from an epidermoid carcinoma of the lung in a 47-year-old Caucasian male. The line can form epidermoid carcinomas in nude mice and tumours in steroid treated hamsters. Ultrastructure includes numerous microvilli, prominent rough endoplasmic reticulum, lysosomes, lipid inclusions and no virus particles. This cell line contains the ras (K-ras) oncogene.
Species: Homo sapiens, Human
Age: male, 47 years
Tissue: Lung; Derived From Metastatic Site: Pleura
Morphology: Epithelial
Growth Properties: Adherent
Organism: Homo sapiens, Human
Species Cell: Human
application: N/A
Doubling Time: ~37 hours
Biosafety: 1
Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Ratio: 1: 2-1: 4
Renewal: 2-3 times per week
