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Product Description
CFPAC-1 Cell Line | EP-CL-0059 | Elabscience
Background: The cells exhibit ion transport activities consistent with cystic fibrosis and express the product of the CF gene(cystic fibrosis transmembrane regulator, CFTR).CFPAC-1 cells show no effect of cAMP agonists, adenyl cyclase stimulators or phosphodiesterase inhibitors on Cl- flux, but do respond to Ca＋ionophores with increase Cl- efflux.The cells have the most common form of the CF mutation, deletion of three nucleotides resulting in the absence of phenylalanine at position 508.CFPAC-1 cells have epithelial morphology and polarization with apical microvilli, tight junctions and gap junctions.
Species: Homo sapiens, Human
Age: 26 years
Tissue: Pancreas, derived from metastatic: liver
Morphology: Epithelial
Growth Properties: Adherent
Organism: Homo sapiens, Human
Species Cell: Human
application: Transfection host.
Doubling Time: N/A
Biosafety: 1
Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Ratio: 1:2-1:4
Renewal: Every 2 to 3 days
