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Product Description
COLO 320DM cell line | EP-CL-0067 | Elabscience
Background: This cell line was isolated from a moderately undifferentiated adenocarcinoma of the sigmoid colon from a 55-year-old female Caucasian. The cells express serotonin, norepinephrine, adrenaline, adrenocorticotropic hormone (ACTH) and parathyroid hormone. The cells are weak-positive for keratins and vimentin.
Species: Homo sapiens, Human
Age: female, 55 years
Tissue: Organ: Colon; Tumor StAge: Dukes' Type C; Disease: Colorectal Adenocarcinoma
Morphology: Rounded
Growth Properties: Adherent
Organism: Homo sapiens, Human
Species Cell: Human
application: N/A
Doubling Time: N/A
Biosafety: 1
Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Ratio: 1: 3-1: 4
Renewal: Every 2 to 3 days
