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Product Description
COS-1 Cell Line | EP-CL-0068 | Elabscience
Background: The cells contain a single integrated copy of the complete early region of the SV40 genome.
Species: Cercopithecus aethiops
Age: N/A
Tissue: Kidney
Morphology: Fibroblast
Growth Properties: Adherent
Organism: Cercopithecus aethiops
Species Cell: Monkey
application: This is an African green monkey kidney fibroblast-like cell line suitable for transfection by vectors requiring expression of SV40 T antigen. This line contains T antigen, retains complete permissiveness for lytic growth of SV40, supports the replication
Doubling Time: N/A
Biosafety: 2
Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Ratio: 1:2-1:4
Renewal: Every 2 to 3 days
