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Product Description
Detroit 562 cell line | EP-CL-0330 | Elabscience
Background: This cell line was established from pleural fluid of an adult female patient with pharyngeal primary carcinoma. The cells have the type B glucose-6-phosphate dehydrogenase, and are immunoperoxidase staining positive for keratin.
Species: Homo sapiens, Human
Age: female
Tissue: Pharynx: Derived From Metastatic Site: Pleural Effusion
Morphology: Epithelial
Growth Properties: Adherent
Organism: Homo sapiens, Human
Species Cell: Human
application: N/A
Doubling Time: N/A
Biosafety: 1
Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Ratio: 1: 2-1: 4
Renewal: Every 2 to 3 days
![K-562 [K562] Cell Line](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/500x659/products/904783/1196984/elabscience__13538.1643193164__44410.1643194172__90041.1647951641__05208.1664298203.png?c=2 "K-562 [K562] Cell Line")
