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![H-4-II-E [H4-II-E] Cell Line](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/1280x1280/products/904750/1196951/elabscience__13538.1643193164__44410.1643194172__90041.1647951641__97579.1664298196.png?c=2 "H-4-II-E [H4-II-E] Cell Line")
![H-4-II-E [H4-II-E] Cell Line](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/100x100/products/904750/1196951/elabscience__13538.1643193164__44410.1643194172__90041.1647951641__97579.1664298196.png?c=2 "H-4-II-E [H4-II-E] Cell Line")
Product Description
H-4-II-E [H4-II-E] Cell Line | EP-CL-0088 | Elabscience
Background: The line has been cured of Mycoplasma arginini contamination.
Species: Rattus norvegicus, Rat
Age: N/A
Tissue: Liver
Morphology: Epithelial
Growth Properties: Adherent
Organism: Rattus norvegicus, Rat
Species Cell: Rat
application: The line is useful for detecting picogram quantities of planar polychlorinated organic compounds in environmental samples and food extracts.
Doubling Time: N/A
Biosafety: 1
Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Ratio: 1:2-1:4
Renewal: Every 2 to 3 days
![H-4-II-E [H4-II-E] Cell Line](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/500x659/products/904750/1196951/elabscience__13538.1643193164__44410.1643194172__90041.1647951641__97579.1664298196.png?c=2 "H-4-II-E [H4-II-E] Cell Line")
![H-4-II-E [H4-II-E] Cells Complete Medium](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/500x659/products/450556/653638/elabscience__13538.1643193164__44410.1643194172__65701.1648028219.png?c=2 "H-4-II-E [H4-II-E] Cells Complete Medium")
