Product Description
HBL-100 Cell Line | EP-CL-0091 | Elabscience
Background: The epithelial cell line HBL-100 has been derived by E.V. Gaffney and associates fromthe milk of a nursing mother and obtained 3 days after delivery. Although there was noevidence of a breast lesion in the milk donor, and the patient had no family history ofbreast cancer, the karyotype of the recovered cells was abnormal as early as passAge 7.This line was able to synthesize a small amount of lactose and would respond toprolactin or estrogen by producing increased amounts of casein. Electron micrographsrevealed microvilli, tonofibrils and desmosomes.
Species: Homo sapiens, Human
Age: 27 years
Tissue: Breast
Morphology: Epithelial
Growth Properties: Adherent
Organism: Homo sapiens, Human
Species Cell: Human
application: N/A
Doubling Time: N/A
Biosafety: 2
Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Ratio: 1:2-1:4
Renewal: Every 2 to 3 days