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Product Description
Hela S3 cell line | EP-CL-0350 | Elabscience
Background: HeLa S3 is a clonal derivative of the parent HeLa line. S3 was cloned in 1955 by T.T. Puck, P.I. Marcus, and S.J. Cieciura.
Species: Homo sapiens, human
Age: Female,31 years
Tissue: Cervix
Morphology: Epithelial
Growth Properties: Adherent
Organism: Homo sapiens, human
Species Cell: Human
application: N/A
Doubling Time: ~48 hours
Biosafety: 2
Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Ratio: 1:2-1:5
Renewal: Every 2 to 3 days
