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![KATO III [KATO 3] Cell Line](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/1280x1280/products/904913/1197114/elabscience__13538.1643193164__44410.1643194172__90041.1647951641__27680.1664298233.png?c=2 "KATO III [KATO 3] Cell Line")
![KATO III [KATO 3] Cell Line](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/100x100/products/904913/1197114/elabscience__13538.1643193164__44410.1643194172__90041.1647951641__27680.1664298233.png?c=2 "KATO III [KATO 3] Cell Line")
Product Description
KATO III [KATO 3] Cell Line | EP-CL-0372 | Elabscience
Background: N/A
Species: Homo sapiens, Human
Age: 55 years adult
Tissue: Stomach, derived from metastatic site: pleural effusion and supraclavicular and axillary lymph nodes and douglas cul-de-sac
Morphology: Spherical
Growth Properties: Mixed, Adherent And Suspension
Organism: Homo sapiens, Human
Species Cell: Human
application: N/A
Doubling Time: N/A
Biosafety: 1
Subculturing: Remove and discard culture medium. These cells grow as a mixture of floating and adherent cells. Sometimes many cells are floating, they can be harvested by centrifugation of medium instead of discarding it. Add 1.0 to 2.0 mL of 0.25% (w/v) Trypsin-0.53mM EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C.
Ratio: 1:2-1:4
Renewal: Every 2 to 3 days
![KATO III [KATO 3] Cell Line](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/500x659/products/904913/1197114/elabscience__13538.1643193164__44410.1643194172__90041.1647951641__27680.1664298233.png?c=2 "KATO III [KATO 3] Cell Line")
![KATO III [KATO 3] Cells Complete Medium](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/500x659/products/450729/653811/elabscience__13538.1643193164__44410.1643194172__83189.1648028239.png?c=2 "KATO III [KATO 3] Cells Complete Medium")
