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Product Description
OP9 Cell Line | EP-CL-0176 | Elabscience
Background: The cells do not produce functional macrophAge colony-stimulating factor(M-CSF) due to an osteopetrotic mutation in the gene encoding M-CSF. The presence of M-CSF had inhibitory effects on the differentiation of embryonic stem(ES) cells to blood cells other than macrophAges.
Species: Mus musculus, Mouse
Age: Embryo
Tissue: Bone marrow/stroma
Morphology: Fibroblast-like
Growth Properties: Adherent
Organism: Mus musculus, Mouse
Species Cell: Mouse
application: OP9 cells can be used to coculture mouse embryonic stem cells(ES cells) to induce the differentiation of embryonic stem(ES) cells into blood cells of erythroid, myeloid, and B cell lineAges. Cocultivation with OP9 does not require exogenous growth factors
Doubling Time: N/A
Biosafety: 1
Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Ratio: 1:2-1:4
Renewal: Every 2 to 3 days
