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![RG2 [D74] cell line](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/1280x1280/products/905019/1197220/elabscience__13538.1643193164__44410.1643194172__90041.1647951641__68647.1664298257.png?c=2 "RG2 [D74] cell line")
![RG2 [D74] cell line](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/100x100/products/905019/1197220/elabscience__13538.1643193164__44410.1643194172__90041.1647951641__68647.1664298257.png?c=2 "RG2 [D74] cell line")
Product Description
RG2 [D74] cell line | EP-CL-0641 | Elabscience
Background: RG2 was submitted to the American Type Culture Collection in March, 1999 at passAge 3 from a culture established from a tumor. This tumor was produced by Drs. A. Koestner and W. Wechsler at The Ohio State University in 1971.
Species: Rattus norvegicus, rat
Age: N/A
Tissue: Brain
Morphology: Glial
Growth Properties: Adherent
Organism: Rattus norvegicus, rat
Species Cell: Rat
application: N/A
Doubling Time: N/A
Biosafety: 1
Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Ratio: 1:2-1:5
Renewal: Every 2 to 3 days
![RG2 [D74] cell line](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/500x659/products/905019/1197220/elabscience__13538.1643193164__44410.1643194172__90041.1647951641__68647.1664298257.png?c=2 "RG2 [D74] cell line")
![RG2[D74] Cells Complete Medium](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/500x659/products/450840/653922/elabscience__13538.1643193164__44410.1643194172__73770.1648028252.png?c=2 "RG2[D74] Cells Complete Medium")
