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Product Description
ST Cell Line | EP-CL-0219 | Elabscience
Background: N/A
Species: Sus scrofa, Pig
Age: 80 to 90 days gestation
Tissue: Testis
Morphology: Fibroblast
Growth Properties: Adherent
Organism: Sus scrofa, Pig
Species Cell: Pig
application: This line is useful for the propagation, assay and isolation of PPV, TGE and swine enteroviruses.
Doubling Time: N/A
Biosafety: 1
Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Ratio: 1:2-1:4
Renewal: Every 2 to 3 days
