Product Description
STO cell line | EP-CL-0220 | Elabscience
Background: This cell line was derived from a continuous line of mouse (Mus musculus) embryonic fibroblasts. The cell line is resistant to for 6-thioguanine and ouabain resistance. Additionally, the cell line is sensitive to HAT and is HPRT negative.
Species: Mus musculus, Mouse
Age: embryo
Tissue: Embryo
Morphology: Fibroblast-Like
Growth Properties: Adherent
Organism: Mus musculus, Mouse
Species Cell: Mouse
application: N/A
Doubling Time: N/A
Biosafety: 1
Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Ratio: 1: 3-1: 4
Renewal: 2-3 times per week