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Product Description
UMNSAH/DF-1 Cell Line | EP-CL-0279 | Elabscience
Background: Foci of non-senescent cells were identified and grown for greater than 30 passAges.No clonal proliferation was observed in soft agar cultures, indicating that these cells were immortalized but not transformed.
Species: Gallus gallus, Chicken
Age: 10 days gestation
Tissue: Embryo
Morphology: Fibroblast
Growth Properties: Adherent
Organism: Gallus gallus, Chicken
Species Cell: Other
application: The cells are useful as substrates for virus propagation, recombinant protein expression and recombinant virus production.
Doubling Time: N/A
Biosafety: 1
Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Ratio: 1:2-1:4
Renewal: Every 2 to 3 days
