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Product Description
Human ADH (Antidiuretic Hormone) ELISA Kit | E-EL-H0272 | Elabscience
Type: Competitive
Assay Time: 2.5h
Sensitivity: 9.38pg/mL
Detection Range: 15.63~1000pg/mL
UniProt ID: P01185 (20~28aa)
Target Name: ADH
Target Synonym: AVP, ARVP, AVP-NPII, AVRP, VP, Vasopressin, Arginine vasopressin
Test Principale: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human ADH. During the reaction, Human ADH in samples or Standard competes with a fixed amount of Human ADH on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human ADH. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The concentration of Human ADH in the samples is then determined by comparing the OD of the samples to the standard curve.
Specifivity: This kit recognizes Human ADH in samples. No significant cross-reactivity or interference between Human ADH and analogues was observed.
Research Areas: Neuroscience, Signal transduction
Storage: An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received.
