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Product Description
Human Ang-Ⅱ (Angiotensin Ⅱ) ELISA Kit | E-EL-H0326 | Elabscience
Type: Competitive
Assay Time: 2.5h
Sensitivity: 18.75pg/mL
Detection Range: 31.25~2000pg/mL
UniProt ID: P01019 (34~41aa)
Target Name: Ang-Ⅱ
Target Synonym: Angiotensin-2
Test Principale: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human Ang-Ⅱ. During the reaction, Human Ang-Ⅱ in samples or Standard competes with a fixed amount of Human Ang-Ⅱ on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human Ang-Ⅱ. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The concentration of Human Ang-Ⅱ in the samples is then determined by comparing the OD of the samples to the standard curve.
Specifivity: This kit recognizes Human Ang-Ⅱ in samples. No significant cross-reactivity or interference between Human Ang-Ⅱ and analogues was observed.
Research Areas: Cancer, Cardiovascular, Metabolism, Signal transduction
Storage: An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received.
