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Product Description
Human GC (Glucagon) ELISA Kit | E-EL-H2237 | Elabscience
Type: Competitive
Assay Time: 2.5h
Sensitivity: 18.75pg/mL
Detection Range: 31.25~2000pg/mL
UniProt ID: P01275
Target Name: GC
Target Synonym: GCG, GRPP
Test Principale: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human GC. During the reaction, Human GC in samples or Standard competes with a fixed amount of Human GC on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human GC. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The concentration of Human GC in the samples is then determined by comparing the OD of the samples to the standard curve.
Specifivity: This kit recognizes Human GC in samples. No significant cross-reactivity or interference between Human GC and analogues was observed.
Research Areas: Cancer, Metabolism, Developmental biology, Signal transduction, Stem cells
Storage: An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received.
